To further validate our methodological framework, we compared our results with synergy predictions based on the combination index method [12], [32], [33].
However, the "protein evolution" experiment presented to validate the methodological development was judged to be of limited interest.
We used an expert panel consensus to validate proposed methodological adjustments and to determine their feasibility and cost.
Nevertheless, additional larger-scale, multicenter studies are needed to externally validate our methodological approach and the results of the present study.
Thus, in order to validate the methodological approaches used in this study, it would be very useful to compare the two case definition strategies with a gold-standard like a valid diagnosis.
To better characterize the amoCAB products obtained from the environmental sample and to validate the methodological approach, clone libraries were prepared with the following PCR products: amoC58f/amoA349r, 305F/amoA-2R, amoA34f/amoBMr, amoA34f–amoB1179r, amoandF/amoA802f/amoBMramoA802f/amoBMr (primer combinations 1, 5, 7, 8, 10, and 13 in Fig. 5).
We designed this study to re-validate some methodological parameters of the radio-enzymatic assay, including phenomena of residual, non-carnitine radioactivity present in some assay mixtures of food samples.
The feasibility of the relative recall approach we have described for validating novel methodological filters may depend on the availability of a number of systematic reviews using the methodology of interest.
SPA-810 has been validated in previous methodological and clinical studies on HSP70 induction [ 22, 35].
