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After quantitative detection, the intensities of each spot were normalized by total valid spot intensity.
The quantity of each spot was normalized by total valid spot intensity.
Further, spot intensities were normalized for total quantity in the valid spot count mode (PDQuest 7.1 software) and given in ppm.
All gel images were normalized to the total valid spot volume of each gel and included for statistical group matches as described below.
Slides were global median normalized so that the median of the median of ratios of every valid spot is equal to 1.
The intensity of each spot was normalised by total valid spot volume and was reported as a relative value in ppm.
Similar(52)
Normalization of each individual spot was performed according to the total quantity of the valid spots in each gel, after subtraction of the background values.
The individual protein spot quantity was normalized as follows: the raw quantity of each spot in a member gel was divided by the total quantity of the valid spots in the gel, and normalized spot intensities were expressed in ppm.
Spots were detected, matched and normalised using the parameter "total of all valid spots".
We then retained 7676 valid spots throughout the 48 membranes and 12 samples studied.
At this point, genes represented by less than 2/3 valid spots were removed.
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