Isolated bacterial DNA from canine faecal samples obtained during the feeding of one of the three specialized diets was used for %G + C profiling and sequencing of valid fractions (referred to herein as fractions 5, 10 and 14) from the %G + C profile.
As result we obtain the exact closed forms for the linear and nonlinear effective elastic moduli, which are valid for any volume fraction of the core embedded in the external shell.
We estimated the fraction of valid tests of LD that showed significant nonrandom association within and between each region.
We estimated the fraction of valid tests of LD that were significant between each region and the gene arrangement type.
Somewhat surprisingly, the high fraction of valid ARFs also carried over for those of the reverse complement strand.
The question then arises of how best to calibrate, when data contain a significant fraction of valid negative responses.
The average fraction of valid ARFs for the TR-proteins in N. vectensis is disproportionately high (37.5%), when considering only the size distribution of these proteins.
This would explain the lower fraction of valid ratios in the direct comparisons, where a number of transcripts having an expression level near detection cutoff in one tissue would eliminate those genes from the analysis when labeled with Cy3.
As shown by Murcray et al., this two-step method can be almost twice powerful than the traditional one-step procedure if the G-E independence assumption is valid for a large fraction of G-E combinations under study.
As noted, these considerations are valid only for the fraction of Arf and COPI that participate in vesicle formation at the rim, rather than for the total Golgi Arf and COPI pool; however, the available evidence indicates that a sizable fraction of the Golgi-associated Arf/COPI is located at the cisternal rims (Oprins et al., 1993 ; Weidman, 1995 ).
On average, arrays hybridized with Cy5-labeled targets derived from individual tissues showed lower non-specific background and produced a slightly larger fraction of valid intensity measurements when compared to Cy3-labeled targets derived from the same RNA samples (data not shown).
