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Electrons liberated are from a field emission source that generates a high electron field gradient in the high vacuum column.
The combined extract was subjected to chromatography on a reversed phase C18-flash vacuum column (5×100 cm) and sequentially eluted with (water/acetonitrile 70∶30, acetonitrile, methanol, dichloromethane and hexane).
The extract was subjected to vacuum column chromatography and eluted stepwise with 100% hexane, hexane-ethyl acetate, ethyl acetate-methanol, and 100% methanol.
Part of FPR (150 g) was submitted to silica gel 60 (0.04-0.063 mm, 120 g) vacuum column chromatography using as eluent, hexane, hexane/CHCl3 1:1 mixture, CHCl3 and CHCl3/MeOH.
The organic residue of Topolia (SP4, 223 mg) was subjected to vacuum column chromatography silica gel, using cyclohexane with increasing amounts of EtOAc as mobile phase, to yield 10 fractions (SP4a SP4b).
The organic residue of Aptera (SP1, 607 mg) was subjected to vacuum column chromatography on Sephadex LH-20, using cyclohexane/CH2Cl2 (1:4 v/v), CH2Cl2/acetone (3:2 v/v) and CH2Cl2/acetone (1:4 v/v) as mobile phase, to yield 11 fractions (SP1f1 SP1f11).
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Of course computer data storage and retrieval is different from audio recording and playback, requiring accurate positioning (both forwards and backwards) to arbitrary spots and therefore much fancier motors, not to mention stress relief for the tape itself, accomplished with vacuum columns (invented by IBM).
The integration heat was favored using an operation pressure for extractant regeneration column EC1 of 1.3 bar because the condensation heat of EC1 was applied in the boilers of vacuum columns AC and WC.
The resulting solution was injected (5 μl) onto a 1200 series LC system (Agilent Technologies), consisting of 1200 series binary pump, autosampler, vacuum degasser, column compartment and control module, with a Luna 3 μm C18(2) 100 Å, 100 mm × 2.00 mm column and a SecurityGuard cartridge kit (Phenomenex, Macclesfield, UK) as a guard column.
One is a standard type used oil, while the other is a residue of a vacuum distillation column.
This is followed by a flash dram and a vacuum distillation column for further purification of the product.
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