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Only after returning to the lab did she recognize the significance of an apparently mundane fact: The other researchers in the lab all happened to wear eyeglasses, which partially shielded them when they used the UV light box.
After electrophoresis, the gel was placed on a UV light box and a picture of the fluorescent ethidium bromide-stained amplified PCR product was taken with a camera (gel documentation system).
Or consider this example: One Friday afternoon, in her early days as a research technician at a major cancer research center, a researcher in a relatively new lab had just completed a gel run and was cutting out the band of purified DNA on an ultraviolet (UV) light box tray.
Gels were run for 2 h at 30 mA, stained with ethidium bromide, and visualized on a UV light box.
Gels were photographed on top of a 280 nm UV light box and images densitometrically quantified with the PC Image J program.
After electrophoresis, the gel was stained with 0.5 µg/ml ethidium bromide for 30 minutes, de-stained in 1 mM MgSO4 for 1 hour, and observed and photographed on a UV light box [41], [42].
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How many people bending over UV light boxes know precisely what kind of eye and face protection they ought to be wearing?
These included formalin-inactivation at 37°C for 3 d, exposure to UV light (UV box equipped with a 3,600 Å light source) for 10 min at room temperature, heat-treatment at 65°C for 2 h, and cross-linking with 0.05% (v/v) glutaraldehyde for 6 weeks at 4°C.
Half of each group (n = 36 each) was exposed to UV irradiation (as UV condition) (254 nm) while being incubated in an anaerobic environment (Modular Atmosphere Controlled System, DW Scientific, Shipley, Yorkshire, UK) at 37°C for 2 h, whereas the other halves (n = 36 each) were kept in a black box avoiding UV light penetration (as UV condition) at the same experimental conditions.
Hands were evaluated under UV light using a Dermalux Box (Bode Chemie GmbH, Hamburg, Germany) before handrub application to make sure that no fluorescent dye was present before the application.
Hands were evaluated under UV light using a Dermalux Box before the application of the hand rubs to make sure that no fluorescent dye was present before beginning the application, and after the application to determine the presence, size and location of untreated skin areas.
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