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Approaches to generate targeted translocations [ 7, 8], deletions [ 5], or duplications and inversions [ 5] of genomic segments in human cells have been developed by generating two double strand breaks (DSB) utilizing two pairs of engineered ZFN.
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A variety of methods for detecting CTCs have been developed including nested RT-PCR, which utilizes two pairs of PCR primers to amplify a single locus.
Use two pairs of flat nose pliers.
The CDCRGDCFC coding sequence were inserted into adenovirus backbone plasmid (pAdeasy-1-E3) by overlap PCR utilizing the two pairs of RGD-related primers described in Supplementary Table S1.
The primary codec is an H.264/AVC codec and the Wyner-Ziv codec utilizes coarse quantization and two pairs of Turbo codecs.
In order to reduce these false-alarms, this paper proposes a new methodology that utilizes transfer impedances obtained between two pairs of collocated PZT patches instead of the electromechanical impedance obtained at one PZT patch.
Two pairs of primers (Table 2), 113seqFw4 and GFPseqRv3; GFPseqFw3 and 113seqRv4 or 116seqFw4 and GFPseqRv3; GFPFw3 and 116seqRv4, were utilized to amplify the regions involved in recombination.
Most beetles have two pairs of wings.
Two pairs of images captured.
Two pairs of prosthetic components were tested.
I must have two pairs of shoes.
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