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As nonspecific binding of the metal cation to nanoparticles is a basic problem in labeling those systems, investigation of nonspecific binding of gallium-68 to a HPMA polymer was performed with 20 nmol HPMA homopolymer without functionalization (Mn: 12.000 g/mol, 0% DOTA) utilizing the optimized protocol.
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Simulations with the optimized protocol showed better performances than usual clinical protocols.
The quality of the optimized protocol is strictly related to the goodness of the model.
The optimized protocol for the KG-1 cell line is available at http://www.lonzabio.com.
Here we report the optimized protocol that allows the detection of human NumtS.
We next investigated whether the optimized protocol and RNaseH treatment alter RT efficiency and linearity.
cDNA synthesis, labeling and hybridization were performed following the optimized protocol developed by the Sequencing & Microarray Facility at SCRI.
Linearity of the qPCR was not statistically significantly affected by the optimized protocol or RNase H (Table 2).
The optimized protocol was applied to archival FFPE cervical carcinomas and CIN lesions for the detection of HPV DNA.
After hybridization, the microarrays were washed using the optimized protocol (http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf) recommended by Agilent technologies.
When VBM5 or SPM5 was used, many apparent segmentation errors occurred, unlike when the optimized protocol of VBM2 was used.
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