A study utilizing the full-length CYP and CPR mutant isoforms simultaneously expressed in insect Sf9 cells via a baculovirus vector recapitulated the results obtained with the bacterially expressed isoforms (referenced above).
PCR primers were designed with the Primer 3 program [ 44], available for use at the URL http://fokker.wi.mit.edu/primer3/input.htm, utilizing the full-length mRNA sequence predicted from the chicken genome available through ENSEMBL http://www.ensembl.org/Gallus_gallus/Search/Results?species=Gallus_gallus;idx= q=gallus as template.
The quantitative western blot methodology that we have chosen has been cited as the gold-standard for RBP4 determination [ 18], and utilizes the full-length RBP4 protein and monoclonal antibody specific for full-length RBP4 protein, greatly increasing validity compared to numerous prior investigations utilizing immunoassay.
We could not detect expression of ZK524.2c, a WormBase predicted short UNC-13 mRNA, by qPCR; therefore, we utilized the full-length MR cDNA for UNC-13S imaging and an MR minigene (nuIs46) (Nurrish et al., 1999) for electrophysiological recordings and behavior.
Degenerate PCR was utilized to clone the full length cDNA of Theobroma cacao NPR1 (TcNPR1).
Finite element modeling was utilized to illustrate the gradient mechanical properties across the full length of the mesh and lay the foundation for future computational development work.
This finding implies that truncated TcdA utilizes other receptor structures for cellular uptake than the full length toxin.
One begins at the same initiation site as the full length LKB1 mRNA, but utilizes an alternative splice site after exon 8 leading to a different C-terminus (Towler et al., 2008).
Transgenic mice with endothelial-targeted overexpression of Nox2 were generated utilizing a tie 2 promoter/enhancer construct [ 26] containing the full length human Nox2 cDNA sequence.
Adenoviruses expressing the full length Prnp gene (Ad-Prnp) were utilized for overexpression of the Prnp gene in PrPC knockout hippocampal neuron cells.
(iii) Realistic dataset with fixed length (RealFix dataset): In order to utilize the full potential of machine learning techniques, we fixed the length to 20 residues as most epitopes have length up to 20 residues.
