As a negative recombinant antibody control for EGFR wt and EGFRvIII, we also used RAbCD133, which utilizes the same expression vector as RAbDMvIII but is directed against CD133 and recognizes neither EGFR wt nor EGFRvIII.
The expression vector was transformed into E. coli C43 DE3.
We thank Professor Jeremy M Tavere and Professor Gavin Welsh for the expression vector pcDNA3 ECFP-DEVD EYFP.
The expression vectors utilized were pEGFP-C (Clontech), pFLAG-CMV 6 (Sigma), pDNR-CMV (Clontech), pQE-30 (Qiagen) and pTNT (Promega).
The expression vectors pET-20b (Novagen) were employed as cloning vector and expression vector.
To ensure that the result above was not due to an artifact of the cell transfection process, we examined CAG cells that had been transfected with the cDNA for heparanase utilizing a different expression vector (pIRES2).
Utilizing pCMV-Myc expression vector, PDCD4 construct without wild 3'-UTR was generated.
For these studies we utilized an expression vector encoding Akt1 devoid of its PH domain and a C-terminally fusion of a farnesylation sequence.
Due to the high output of TMV CP and facile extraction of viral particles from systemically infected plants, TMV has been intensively utilized as an expression vector to synthesize commercial foreign peptides in tobacco [2].
Utilizing the multi-system vector pBEV, a high-throughput process was developed with expression in deep-well blocks and purification in micro-titer plates enabling the identification of expression and solubility in both E. coli and insect cells.
