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For instance, PTMap [ 43] utilizes sequence alignment to localize sites of PTMs on target proteins while substantially reducing false-positive identifications based on unmatched peaks.
The sequence was rapidly determined using a previously described approach that utilized a PCR-based method and employed universal primers, designed on the scaffold of multiple sequence alignment of chloroplast genomes.
A large proportion of these algorithms utilize sequence conservation within a multiple sequence alignment (MSA) of homologous sequences to identify intolerant substitutions, for example, a substitution falling within a conserved region of the alignment is less likely to be tolerated than a substitution falling within a diverse region of the alignment (see reference [ 40] for a review).
Sequence alignment of Piezo orthologues.
(b) Multiple sequence alignment of NucS sequences.
b, Sequence alignment of TcB sequences.
c, Sequence alignment of TcB sequences.
Multiple sequence alignment of human PADs.
(a) Sequence alignment of TomB/YmoB proteins.
Sequence alignment of Pvs25 and P28 molecules.
Multiple sequence alignment of gasdermin family members.
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