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High-fidelity fusion PCR utilizing sequence overlap between the two synthetic gene halves allowed the production of a DNA fragment that was able to recombine the full-length psbA2 gene into the Synechocystis chromosome at an ectopic (non-native) location.
The targeted introduction of mutations utilizing sequence specific transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (RNA-guided nucleases, RGNs) has revolutionized reverse genetic approaches in numerous model organisms.
The feasibility of utilizing sequence features that can be viewed as evolutionary changes in the sequence occurring in the operating taxonomic units has received considerable interest.
These results in their entirety demonstrate the feasibility of utilizing sequence information for the prediction of interaction specificity, and represent a significant step forward towards a system-level understanding of entire signaling networks in bacterial organisms.
In addition, by utilizing sequence data on a portion of individuals in the study, we are able to have a perfectly matched reference panel, in terms of linkage disequilibrium, without relying on the assumption that the HapMap populations represent our study population (as HapMap based haplotypes are the current standard reference data used for genotype imputation).
A variety of population genetics approaches exist to quantify adaptive evolution utilizing sequence divergence between species.
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The bottom part of the figure shows how the same video clip is organized utilizing sequences created from the watching position of the clients.
We report here on the application of a massively parallel sequencing approach utilizing sequencing-by-synthesis [ 10] as an efficient approach to generate ESTs.
Previous analyses utilizing sequenced BAC clones (23) of the 17q21.31 inversion localized the inversion breakpoints to homologous copies of the LRRC37 core duplicon (24).
Comparative mapping utilizing sequences of SNPs flanking PHS resistance QTL enabled identification of candidate genes and regions in Brachypodium and rice.
Since these observations were unexpected, the phylogenetic history of nifH was reconstructed utilizing sequences from species of the orders Rhodospirillales and Rhizobiales.
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CEO of Professional Science Editing for Scientists @ prosciediting.com