Suggestions(1)
Exact(4)
cDNA templates (diluted in a 1 10 ratio) were used to perform PCR quantification (utilizing conditions described above).
Utilizing conditions (ii)–(iv), we know from (2.30) that (2.31).
Animals with a clinical score of 0.5 or above were used in gene therapy experiments and were administered 50 μg of DNA intramuscularly in 60 μl at three sites and were electroporated utilizing conditions described above.
With proper selection of the replication origin and antibiotic-resistance marker, we initially electroporated methylated DNA into C. pasteurianum at a low efficiency of 2.4 × 10 transformants μg-1 DNA by utilizing conditions common to other clostridial electroporations.
Similar(56)
Species that are good at colonizing can utilize conditions provided by urban heat islands to thrive in regions outside of their normal range.
Lastly, Bengtsson and coworkers utilized conditions in which musical improvisations were generated and then subsequently reproduced by memory.
We utilized conditions common to clostridial electrotransformation procedures (Table 1) and M.FnuDII-methylated DNA.
To provide optimal conditions for subsequent Flp-based gene insertion, we utilized conditions which in our hands consistently allow insertion of >1 vector in transfected HEK-293 cells.
The second system utilized conditions of rapid combustion and exposure of rats to the total products of combustion for a period of 4 hr.
Similarly, a sufficiently small subfault, which can be regarded as a point source, is utilized (condition 2).
Therefore, to determine whether TNBC cells sensitive to EGFR TKIs have the proper protease(s) to mediate cleavage of pro-HGF, activation of Met, and subsequent EGFR/Met crosstalk, we utilized conditioned media from a human fibroblast cell line engineered to express human HGF (RMF-HGF) [ 18, 19].
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