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Samples were run in triplicates and the efficiency of each primer was calculated utilizing an internal standard control.
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The LC separation and the ESI settings of the Esquire instrument were optimized utilizing a dibenzyl oxalate as an internal standard.
Utilizing the heavy peptide signals as an internal standard (by calculating the peak area ratio, or in log scale, subtracting the logs of heavy peptide measurements), removes this source of experimental variability.
4-morpholineethanesulfonic acid (MES), the buffer compound in the oMM medium with constant concentration and not utilized by yeast, was used as an internal standard (IS) for concentration normalization.
Supelco 37 Component FAME Mix (Sigma-Aldrich Co., St . Louis MO, USA) was utilized as a quantitative standard, and pentadecanoic acid (Sigma-Aldrich Co). was used as an internal standard.
Supelco 37 Component FAME Mix (Sigma-Aldrich Co., St .Louis, Missouri, United States) was utilized as a quantitative standard and pentadecanoic acid (Sigma-Aldrich Co., St .Louis, United States) was used as an internal standard.
Inositol was used as an internal standard.
28S rRNA served as an internal standard.
Vigabatrin was used as an internal standard.
Nicotinamide is used as an internal standard.
Norvaline is used as an internal standard.
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