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E. coli libraries expressing mutant LovD were obtained, and the whole-cell activities of converting monacolin J to simvastatin were assayed by utilizing an agar-based diffusion screening assay.
Being an agar-based method, contamination can be easily monitored, resulting in more rigorous quality control in the early stages of hit identification.
Disc diffusion is simple and inexpensive agar-based method which enables the determination of activity of different substances against microrganisms.
Here, we have clearly enriched for transcription complexes utilizing a LIS-based method, suggesting that scaffold attachment may be functional.
Vegetarians can make this dessert using an agar-based gelling agent.
The advantages of the liquid-based screen developed are listed along with the limitations of an agar-based assay.
To fabricate synthetic gene constructs we utilized a PCR-based method of gene synthesis [24] that involves parsing the DNA sequence into a set of small overlapping oligonucleotides.
Therefore, to assess localization of cathepsin B to caveolae-enriched fractions of SUM149 3D cultures, we utilized a detergent-based method that separates TS from TI membrane components.
In this study, to investigate genetic variations affecting the response to selenium, we utilized an innovative statistics-based method previously reported by us [31].
PAT utilizes a home-based visitation method in which a trained parent educator goes to the home at least once a month.
To bypass these problems, we used an agar-plate-based method to replace the liquid-based outgrowth (Fig. 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com