Sentence examples for utilizing a specific sirna from inspiring English sources

The role of PLD1 was investigated by functional genomics, utilizing a specific siRNA to silence the expression of PLD1.

Control non-silencing siRNA (siCont) (MISSION siRNA Universal Negative Control), FITC-labeled non-silencing siRNA and human VEGF-A specific siRNA(11) were purchased from Sigma-Aldrich (St . Louis MO, USA).

1.5×106 cells (triplicates) were transfected with control siRNA (Alexa 488 conjugated siRNA) and a specific siRNA targeting BAT3 (Qiagen) using quantifect transfection reagent method (Qiagen).

WSB1 expression was knocked-down in cultured cells with a specific siRNA.

A specific siRNA to ASCL1 (AsiRNA/ARNand) and one non-specific Control siRNA were transfected to BON1 cells.

To knockdown Cx43, these cells were transfected with a specific siRNA for Cx43 (Cx43-siRNA) or a non-target siRNA (NT-siRNA) used as control.

B7-H1 expression was inhibited in MDA-MB-231 cells using a specific siRNA (CD274 siRNA ID = s26547 and siRNA ID = s26548, Ambion, Austin, TX, USA).

We reduced BRD4 expression utilizing a well-characterized siRNA and measured BRD4 mRNA levels via qRT-PCR analysis.

For generation of A-RAF specific siRNA we used "X-tremeGENE siRNA Dicer Kit" (Roche).

Here, we utilized a functional siRNA kinomic screen to identify factors involved in SMC-resistance.

To test whether cdr2 may be important in mitosis, we performed cdr2 knockdown utilizing a pool of 4 cdr2-specific siRNAs that reduced cdr2 mRNA or protein levels (Figs. 3A, 3B, S3) to less than 10% of control siRNA-treated HeLa cells.