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We extended the analysis of these compounds to PptT utilizing a recently published fluorescence polarization (FP) assay that measures modification of a CP target with a synthetic fluorescent CoA analogue (Table 1).
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Secondly, we also utilized a recently published SOLiD sequencing dataset obtained from high-throughput RNA sequencing (RNAseq) of mouse embryonic stem cells [ 16].
To further explore the correlation of p21 gene expression level with clinical outcome in breast cancer patients, we utilized a recently published gene profiling database of breast cancer patients to assess p21 gene expression in overall survival (OS) and distant metastasis-free survival (DMFS) outcomes [ 34].
To test this model, we utilized a recently published parasite line in which the export of parasite proteins into host erythrocytes, including establishment of nutrient permeability pathways, can be conditionally regulated with the synthetic small-molecule ligand trimethoprim (TMP) (Beck et al., 2014).
To compare the optimal result of this design flowsheet with a recently published separation method utilizing two reactive-distillation columns, our proposed design is much more economical with 61.4% savings in total annual cost and 64.6% savings in annual operating cost.
Furthermore, in a recently published collaborative study utilizing this antibody, it was shown DAF-deficient T cells are required for induction of disease with the intact version of this antibody [34].
We also took advantage of a recently published miRNA prediction tool utilizing integrated sequence-structure motifs to identify miRNA precursors (ss-motifs [ 28]).
The resulting closed system of ODEs (ordinary differential equations) represents an extension to a recently published method of moments which utilizes least-square approximations of factors of the PDE over orthogonal polynomial bases.
RBF-TSS employs a recently published clustering algorithm for initialization that utilizes the large number of available background samples found within genomic DNA [16].
In a recently published study, a similar scaffolding approach was utilized to present the poorly immunogenic gp41 4epitopetope, which is located adjacent to the 2F5 epitope in the viral Env [42].
This assay utilized Saccharomyces cerevisiae strain BF3505 transfected with the receptor plasmid YEPE10 and followed a recently published protocol [ 45].
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