Sentence examples for utilizing a random hexamer from inspiring English sources

One microgram isolated total RNA was DNAse treated (Promega) and used as template to generate complementary DNA (cDNA) utilizing a random hexamer primer [p dN 6] and MMLV reverse transcriptase (Promega).

To obtain first strand cDNA pools, reverse transcription was carried out using SuperScript IV (Thermo Fisher Scientific) with a random hexamer and oligo dT primer, following the manufacturer's instructions.

cDNA was synthesized using a random hexamer primer.

One microgram of total RNA was reverse transcribed with avian myeloblastosis virus reverse transcriptase (Promega, USA) and a random hexamer.

First-strand cDNA was synthesized using a random hexamer primer using the mRNA fragments as templates.

First-strand cDNA was synthesised from 1  μg of total RNA using a random hexamer primer.

First-strand synthesis was synthesized with a random hexamer and SuperScript II (Life Technologies Corp., Carlsbad, CA).

First strand cDNA was synthesized using a random hexamer primer and M-MuLV reverse transcriptase.

The synthetic library was amplified with three commercially available DNA polymerases using an anchored primer with a random 3' hexamer end.

For cDNA synthesis, RNA (5 μL), a random hexamer, and a SuperScript III Kit (Invitrogen, Carlsbad, CA, USA) were used.

cDNA was reverse transcribed using a cDNA Synthesis Kit (BioLine, London, UK) with a random hexamer primer mix.