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In order to test whether Map3k4 haploinsufficiency is absolutely required for Tas, or whether loss of other loci in the T hp deletion is also sufficient to generate a sex reversal phenotype, we performed a gain-of-function experiment utilizing a previously reported Map3k4 BAC transgenic line, c06 (28) (Fig. 3), and lines harbouring an independent Map3k4 BAC, k10 (data not shown).
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To add to the complexity, many publications utilize a previously reported model to extend the characterization of the phenotype.
Hence, the two mutants were purified in their apo form using a previously reported protocol, which utilizes hydrophobic affinity chromatography to separate apo and holo hemophores.
The purpose of this study was to analyze longitudinally the longer term results of a previously reported cohort of patients utilizing a cementless modular acetabular component with a MoM bearing.
Patient data and MIBG myocardial scintigraphy images collected as part of a previously reported clinical study were utilized in the present analysis [ 10].
16 17 18 19 20 21 One study, from the US, utilized a pre-post observational design, 15 and one paper was a subanalysis of a previously reported randomized controlled trial 22 (table 1).
The approach is illustrated with a previously reported case study.
following a previously reported procedure.
We utilized the WT-Pico method to amplify RNA extracted from the C. elegans nervous system by the mRNA-tagging method and compared microarray data from this sample to a previously reported pan-neural profile generated with IVT amplification [ 8].
We report on the outcomes of Reverse total shoulder arthroplasty (RSA) utilizing a previously unreported prosthesis, the SMR Modular Shoulder System (Systema Multiplana Randelli, Lima-LTO, Italy).
We have also included a quantification of the activation energy for Hsp90 homologs (yHsp90 and Grp94) in Figure 4 figure supplement 2, utilizing previously reported rates found in Frey et al 2007.
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