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The aim of the present study was to explore the early kinetics of the HSV-1 infection and the consecutive host-dependent innate immune responses after 12 hours and each of the first four days post infection (dpi) by utilizing a previously described DA rat model of lethal HSE [5].
The potential of CPC derived cardiomyocytes to form functional gap junctions was determined by utilizing a previously described in vitro NRVM co-culture assay [17], [18] which allows the observation of action potential propagation by the means of optical mapping over a macroscopic (3 culturelture area [18].
Utilizing a previously described cohort of 458 individuals with hypertriglyceridemia (defined as fasting plasma triglyceride levels above the 95th percentile) and 333 control individuals with normal plasma triglyceride levels, 30 individuals (24 cases, 6 controls) harboring a total of 18 rare (minor allele frequency <0.01) GCKR variants were identified by targeted exon sequencing (8).
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For the 293T and MCF7 cells we utilized a previously described BACH2 sgRNA and observe on-target methylation that is comparable to their published results13 (Fig. 3b, c).
In order to prospectively identify and capture data on consecutive post-cardiac arrest patients, we utilized a previously described 24-hour per day, 7-day per week paging system [6, 16].
We utilized a previously described C2C12 line (CUGBP1 KD) in which CUGBP1 is stably knocked down through expression of an shRNA targeting the 3'UTR [15].
To confirm hypoxia induction of TLR2 and TLR6 in vivo, we utilized a previously described murine model of ambient hypoxia [26] [30].
To do this, we utilized a previously described constitutively active variant of CSF-1R (CA-CSF-1R) and CA-CSF-1R with a tyrosine to phenylalanine substitution at Y561 (CA-CSF-1R Y561F), the recruitment site for SRC and SFKs [8].
We utilized a previously described iterative approach for large-scale RLS analysis [14]: each deletion mutant was initially assayed in strains derived from the MATα ORF deletion collection and, in cases where a statistically significant (p<0.05) increase in median RLS was observed, RLS was determined for independently derived isogenic cells obtained from the MATa ORF deletion collection [14].
The authors utilized a previously described cadaver model [ 22].
We utilized a previously described protocol to evaluate ubiquitination (Li et al, 2003).
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CEO of Professional Science Editing for Scientists @ prosciediting.com