Phosphorylated Foxo3a was identified utilizing a polyclonal rabbit-anti-rat Foxo3a (phosphor S253) antibody (Abcam), followed by HRP-conjugated goat-anti-rabbit IgG (Santa Cruz Biotechnology).
To detect the N1 protein a polyclonal rabbit anti avian influenza A neuramindase (Abcam, ab21304) was utilized.
We have raised a polyclonal rabbit serum in order to detect the neu protein product in tissue sections of tumors.
The antibody was a polyclonal rabbit antibody, which recognized fibrinogen (ref. F0111, Dako SA, Trappes, France).
The first combination is a polyclonal rabbit anti-human IgG with a F ab)′2fragment human IgG as the antigen.
CD105, a polyclonal rabbit antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), was used as a marker for rat stem cells.
(iii) A polyclonal rabbit anti-human IL-2 antibody caused nearly complete inhibition of DNA synthesis by B cells activated by ionomycin and PMA.
The Mr 95,000 glycoprotein has been purified by antibody affinity chromatography and a polyclonal rabbit antiserum raised that exhibits identical specificity to the monoclonal antibody.
S. Typhimurium was stained using DAPI and/or a polyclonal rabbit α-Salmonella O-1,4,5,12 8) O-1,4,5,12 8ifco) antiserumlonal goat α-rabbit-Cy5 conjugate (Fig. 2).
A polyclonal rabbit anti-MAD2B antibody was used as described before [15].
For endogenous control a polyclonal rabbit anti-human GAPDH (R&D Systems Inc).
