Sentence examples for utilizing a polyclonal antibody from inspiring English sources

Expression of UGT1A01 protein was also detected by a Western blot utilizing a polyclonal antibody developed against the human UGT1A family.

Similar results were obtained utilizing a polyclonal antibody recognizing TIRC7 [1] which also co-precipitated HLA-DR alpha chain as confirmed by protein sequencing (data not shown).

Albumin concentration of medium samples was analyzed by an enzyme-linked immunosorbent assay (ELISA), in triplicate, utilizing a polyclonal antibody to rat albumin (Cappel Laboratories, Aurora, OH).

We have utilized a polyclonal antibody raised against highly purified human plasma BuChE that has previously been demonstrated to be effective in immunoprecipitating human BuChE [38].

This RIA utilizes a polyclonal antibody specific for the VGF-derived C-terminal peptide AQEE30 (VGF peptides are designated by the four N-terminal amino acids and length), so detects AQEE30 and the N-terminally extended peptides that contain this sequence, TLQP62 and NAPP129 [ 38].

IHC analysis for cyclin L2 (CCNL2) was performed in 21 NP and 29 P CNBs tissues utilizing a polyclonal anti-human cyclin L2 (CCNL2) antibody (Novus Biologicals, Cambridge, UK) at a concentration of 5μg/ml.

Phosphorylated Foxo3a was identified utilizing a polyclonal rabbit-anti-rat Foxo3a (phosphor S253) antibody (Abcam), followed by HRP-conjugated goat-anti-rabbit IgG (Santa Cruz Biotechnology).

Immunostaining for activated (cleaved) caspase-3 utilized a rabbit polyclonal antibody (AB3623, Merck Millipore) at a 1 1,000 dilution.

Confirmation of in situ localization of NOS in tumours utilized a rabbit polyclonal antibody for iNOS.

Since the monoclonal UCH-L1 antibody utilized in this experiment recognized only the full-length 25-kDa form of UCH-L1, we incubated a parallel blot with a polyclonal antibody for UCH-L1 to visualize additional cleavage fragments.

These assays used a polyclonal antibody against hCG dimer.