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The particle concentration of the GNPs (15 nM) was determined based on Beer's law utilizing a molar extinction coefficient of 2.43 × 108 M−108m−10839].
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LPO in units/mg protein was computed with a molar extinction coefficient of 1.56 × 105 M-.
Content of TBARS was expressed in micromoles of malondialdehyde using a molar extinction coefficient 1.55 × 105 cm−105−105
Reaction rates were calculated using a molar extinction coefficient of 36 mM/cm.
Concentration was determined spectrophotometrically using a molar extinction coefficient of TAMRA (80,400 M−1 cm−1) at 547 nm.
A molar extinction coefficient of 1.56 × 105 M−1 cm−1 was used for calculations.
Ascorbate consumption was monitored by spectrophotometry at 290 nm, with a molar extinction coefficient of ε = 2.8 mM−1 cm−1.
The absorbance decay of H2O2 was monitored by spectrophotometry at 240 nm, with a molar extinction coefficient of ε = 39.4 mM−1 cm−1.
The reaction was followed spectrophotometrically by employing the change in the absorbance at 420 nm and a molar extinction coefficient of ε 420, 36,000/M/cm.
AChE activity was calculated using a molar extinction coefficient 1.36 × 10 − 4 M − 1 cm − 1 and expressed in μmol− 1 min − 1 mg protein− 1.
GSH content was calculated using a molar extinction coefficient of 13.6 × 103 M−1 cm−1 and reported as nM/mg protein (Moron et al. 1979).
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