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The purpose of the present study is to report our experience in with MRE utilizing a modified protocol adapted to maximize patient and fetus safety in the context of suspected CD or CD exacerbation in a tertiary referral center setting.
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Preparation of plasmid DNA was performed utilizing a modified alkaline lysis protocol followed by purification through anion exchange column (Qiagen, Valencia, CA).
For cytokine detection restimulation was performed using a modified protocol.
Nuclei were isolated using the Sigma Nuclei EZ Prep Nuclei Isolation Kit using a modified protocol.
Cell extracts were prepared using a modified protocol from Rigaut et al. [30] and the NCRR Yeast Resource Center.
Gels were blotted and treated with antibodies using a modified protocol [37].
SeMet-substituted XLF441 was expressed by using a modified protocol.
We used a modified protocol enriched with the CD133 antibody.
Mitochondria were subfractionated following a modified protocol [ 36].
The HOP protocol used follows a modified protocol from Flesch et al. [ 25].
Insolubility of the human αSynuclein aggregates was demonstrated utilizing a modified version of a PK digestion and immunohistochemistry protocol [ 12].
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