Sentence examples for utilizing a linear gradient from inspiring English sources

The column was equilibrated in 85% A/15% B prior to the injection of the sample, and upon injection this composition was then changed to 60%A/40%% B over 30 min utilizing a linear gradient followed by changing to 50%A/50%% B over the next 10 min, and then, the concentration was returned to 85%A/15%% B over the final 10 min.

(22) All labeled compounds were purified by reverse phase-high performance liquid chromatography (RP-HPLC) utilizing a C4 semipreparative column (Waters) with a linear gradient of 0 100% acetonitrile with 0.1% trifluoroacetic acid over 40 min. Acetonitrile, TFA, and water were removed from fractions containing nisin or vancomycin by rotary evaporation followed by lyophilization.

A linear gradient of 78% mobile phase B to 48% mobile phase B in 75 min was utilized.

Linear sucrose gradients were performed with a linear gradient maker.

Elution was done applying a linear gradient of NaCl.

A linear gradient was used in all measurements.

A linear gradient of 100∶0 (A∶B) to 56∶44 was run over 20 minutes followed by a linear gradient of 56∶44 to 50∶50 over the next 10 minutes and finally a linear gradient to 0∶100 from 30 45 minutes.

Elution employed a linear gradient from 10% to 70% buffer B in buffer A over 16 minutes.

A linear gradient from 0-40% B over 40 min was used.

The TopBK protein was eluted using a linear gradient from 15 to 500 mM imidazole.

A linear gradient was performed for the sample analysis.