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However, utilizing a cutoff of ≥1 to achieve a sensitivity of 97% resulted in a specificity of only 13%.
The trend of increasing specificity with quantitative analysis is consistent with a study comparing initial DaT scan imaging diagnoses with 6-month clinical follow-up (Jennings et al., 2004), which reported an increase in specificity from 80%to100%0% when utilizing a cutoff of 30% decrease in age-corrected putamen uptake from normal controls to define abnormal DaT scans.
Utilizing a cutoff of −17 cm H2O for maximum inspiratory pressure, Conti et al 23 found a sensitivity of up to 0.95 and a specificity of 0.42.
We define high cOPN as any value above the bottom three quartiles of healthy controls (n = 62; quartiles utilized as values are skewed to the right in the adult cohort) and also perform the high cOPN analyses utilizing a cutoff of two SD above the mean for young, healthy controls (mean age 20.2 years; n = 22).
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Similar to a prior study, we utilized a cutoff of ≥16 to represent a high degree of pain catastrophizing [ 19].
Data from samples analyzed by Mascot were compared with ProteomeDiscoverer software (Thermo Scientific) utilizing a cutoff score of 40 and a requirement of two peptides per protein.
Protein secondary and tertiary structures were maintained in the CG simulations by means of an elastic network model utilizing a distance cutoff of 7 Å (force constant of 10 kJ mol−1 Å−2).
We acknowledge that utilizing a BMI cutoff of ≥ 99th percentile has some shortcomings and instability [ 38], but at this time, this cut-off is the most accepted and utilized measure in the field.
Utilizing a high cOPN cutoff of the bottom three quartiles of healthy controls of all ages (n = 62) or 2 SD above the mean for young, healthy controls (n = 22) yielded identical results; the groups of normal versus high cOPN in this study are not altered by the change in the cutoff.
Although these cells were detected utilizing 8q24 and 3q26, a cutoff of 30 abnormal cells prevented these cells from impacting the specificity of these probes.
The remainder of the postscreen library was shown to be inactive toward the receptor, and a clustering approach was utilized to identify 18 unique scaffolds at a cutoff of 25% similarity.
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