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Sequence capture, which utilizes probes to enrich target sequences (e.g. Bi et al. 2013) (Box 1), offers a compromise between minimizing sequencing costs and maximizing sequence information from target regions of the study organism.
The Digene LQ test utilizes probes for 18 HR HPV types (i.e., HPV 16,18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) that are the same as the respective RLB probes, with minor modifications, and are immobilized on color-coded beads.
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Agilent and Codelink both use single probes to target a gene, but the Affymetrix U95Av2 genechip utilizes probe sets consisting of 16 probes to target a gene.
A probe-set frequency pattern is the collection of the normalized frequencies of all utilized probes.
ISH originally utilized probes in the form of radioactively labeled rRNA, visualized by autoradiography [ 1, 2].
The interviewer utilized probes to explore topics related to coping strategies, trauma and family and community relationships in greater depth.
Solid-phase hybridization methods generally utilize probes complimentary to the sequences of interest affixed to a solid support, such as microarrays (7– 11) (Fig. 1A) or filters (12).
In addition, utilizing probe S4, cyanide ion in real industrial samples can be rapidly recognized and quantitated by the naked eye.
A mixed-model approach was chosen to adequately describe between-array variation and still utilize probe-replicates (3 replicates of each probe in each array).
MAS5.0 is a relatively simple algorithm that processes a single chip at a time, utilizing probe position on the chip and mismatch (MM) probes to correct the perfect match (PM) probe signal readout.
Pre-processing involved non-linear background reduction utilizing probe DNA sequences, quantile normalization, and summarization by median polishing [ 8, 9].
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