Sentence examples for utilizes a single pcr from inspiring English sources

Given this scenario, the pentaplex PCR offers tremendous overall advantage over NCI-markers, as it is more rapid, utilizes a single PCR reaction, obviates the need for normal DNA, is less expensive and is highly accurate.

Cycles were performed as follows: denaturation at 95C for 3 min to activate the polymerase, followed by 40 cycles of 95C for 15 s, 60C for 30 s, and 72C for 30 s. Dissociation curve analysis and gel electrophoresis were utilized to ensure that a single PCR product was amplified in each reaction.

TAS was further extended to a single PCR reaction that utilized linker sequences which allowed common target primers and a single set of barcoded primers to be utilized across distinct samples and loci [ 10].

We developed a multiplex PCR system designed to enhance specificity for MAP detection in a single PCR reaction.

A single PCR product was obtained and sequenced (1,954 bp, accession No. AB901085).

The usefulness of a single PCR for the detection of B. henselae and B. clarridgeiae in the blood of cats is questionable and enhancement methods, such as a nested-PCR, should be utilized.

*Y57G11B has two DraI SNPs within a single PCR product.

Site-directed mutagenesis of large (13-kb) plasmids in a single-PCR procedure.

GARP utilizes a single point per grid cell to identify it as present for B. anthracis.

Second, the system is typically orthogonal to endogenous pathways and utilizes a single defined ligase.

In this study, we present a highly customizable method for quantifying copy number and point mutations utilizing a single-color, droplet digital PCR platform.