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This assay utilizes a multiplex PCR technology called Anchored Multiplex PCR for single nucleotide variant and insertion/deletion (indel) detection in genomic DNA using next generation sequencing.
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Polymerase chain reaction (PCR) probe is a multiplex PCR probe targeted at specific insertions.
Using a multiplex PCR assay, X. oryzae pv.
A multiplex PCR amplifying 11 polymorphic markers was designed.
Single PCR was used to confirm primer specificity, and PCR conditions were optimized to construct a multiplex PCR system.
So far, Monilinia diagnosis required a multiplex PCR analysis and gel electrophoresis.
We developed a multiplex PCR system designed to enhance specificity for MAP detection in a single PCR reaction.
The primers were also used to screen a collection of field isolates in a multiplex PCR.
To facilitate their rapid identification, we developed a multiplex PCR assay targeting conserved, species-specific genes.
Firstly, a multiplex PCR method designed to amplify seven genes for use in MLST was developed.
The limit of detection in wastewater was 103 104 cells L−1 with a multiplex PCR protocol.
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