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To determine whether Kif3B affects LR patterning solely because of its role in cilia, we utilized the protocol described in Strategy 3 above, injecting the mutant Kif3B mRNA construct during the early 1-cell stage in biased locations of the embryo.
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A small piece of tail was cut from three weeks old mice and was used for the extraction of genomic DNA utilizing the protocol described in our previous work (39).
The protocol described here may be utilized for the overexpression of other eukaryotic cis-PT.
The assay was performed according to the protocol described previously46,47.
Staining was performed using the protocol described above.
Surgery was performed according to the protocol described above.
The protocol described here should be useful to better describe, quantitatively, each of these steps.
injection, according to the protocol described below.
We followed the protocol described previously [20].
The protocol described here is V.7.
Sample analysis followed the protocols described above.
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