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To determine whether Kif3B affects LR patterning solely because of its role in cilia, we utilized the protocol described in Strategy 3 above, injecting the mutant Kif3B mRNA construct during the early 1-cell stage in biased locations of the embryo.
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Cells were cultured utilizing the protocol previously described[29].
Highly differentiated fetal human RPE cells were grown utilizing the protocol of Maminishkis et al.[ 15]with some modifications [ 3].
The 6 MWT was performed walking a corridor of 100 feet in length utilizing the protocol outlined by the American Thoracic Society ATS [ 1].
A small piece of tail was cut from three weeks old mice and was used for the extraction of genomic DNA utilizing the protocol described in our previous work (39).
It is of interest that these studies utilized the same protocol, yet realized such different rates of hypoglycemia, demonstrating that factors outside of the protocol rather than the glucose target may significantly influence glucose control and hypoglycemia.
For in vitro biofilm formation under anaerobic conditions, we utilized the same protocol as described above with the following exceptions.
The most recent published Japanese cooperative ATLL trial utilized the LSG15 protocol, and included 58 patients with leukemic ATLL, 28 patients with lymphoma type, and 10 patients with unfavorable chronic ATLL [24].
In this study, we utilized the GBS protocol of Elshire et al. (2011) with minor modifications.
For Western blotting, 50 μg of protein was used per sample (extraction from tissue utilized the TriZol protocol).
The application protocol utilizes the following protocol stack for communication between the geolocation database and a master WSD: Application layer (HTTPS); Presentation layer (XML); Session layer (Undefined); Transport layer (TCP); Network layer (IP); Data link (Undefined); Physical layer (Undefined).
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