A sorting strategy that utilized the level of CD45 expression as a discriminator between pre-BI, pre-BII and naïve B-cells and a stringent gating strategy were employed to ensure that the 4 distinct populations of cells were collected with no overlap.
In addition, the predicted value of COF by utilizing the levels of the optimal design parameters (0.5 vol.%hBN, 0.3 vol.% surfactant),as made by the Taguchi optimization method, was consistent with the confirmation test (average value of COF = 0.07215), which fell within a 95% confidence interval (CI).
For the patients with multiple diagnoses, their 25(OH D was counted in each diagnostic category, which might overestimate the prevalence of hypovitaminosis D. The study utilized the lowest level of 25(OH D for patients with multiple measurements, which might result in lower mean level and higher prevalence.
These computer simulations also confirmed that tensegrity principles are indeed utilized at the level of individual molecules, multi-molecular assemblages, and whole living cells.
For the real-time RT-PCR assays, a Kruskal-Wallis test and Dunn's multiple comparisons tests were utilized with the level of significance set at P<0.05.
The two elements utilized included the level of evidence and the clinical impact.
Reagents have traditionally been fed into the impeller region of stirred tank reactors to utilize the high levels of turbulence, improve mixing rates and hence reduce mixing limitations.
Difference equations are utilized to update the levels of calcium in the intracellular and extracellular compartments, incorporating endocrine regulation through the parathyroid hormone.
Flow cytometry was further utilized to quantify the levels of apoptosis at the selected concentration and time point via MTT assays.
The propagation of error and desirability function were utilized to identify the levels of the selected factors that simultaneously optimize all the responses while reducing the variation transmitted to the responses from the variability of the controlled factors.
