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To search for Medaka homologs of Fas, FADD and caspase-8 in the GenBank database, we utilized the BLAST program [ 29] using the human counterparts as a query.
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Nucleotide BLAST for mature sequences was performed utilizing the blastn program with the blastn-short task default parameters, except for word-size which was changed from the default of 7 to 16; in this manner, only nearly identical sequences were identified.
This program utilizes the BLAST searches of a set of sequences encoding the required activity in other genomes to identify candidate proteins in the genome of interest, in addition to genomic context (such as the candidate enzyme being in the same operon as another gene in the pathway) to determine the probability that a candidate enzyme has the required function [29].
The Blast program "blastall" was downloaded from NCBI and used to annotate the sequences.
Blast searches were performed using the blast program available at http://blast.ncbi.nlm.nih.gov.nih.gov
The selected sequences were analyzed through the BLAST program (http://www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
For this purpose, the blast program in NCBI BLAST is included in Follicle Online database.
Their specificity was tested using the BLAST program from NCBI.
Data base searches were carried out with the Blast program.
The sequence similarity was determined by using the BLAST program from the National Center for Biotechnology Information (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
For sequence analysis, the BLAST program from the National Center for Biotechnology Information Web site was used (http: //www. ncbi. nlm. nih.gov/BLAST).
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