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In this work we utilized site directed mutagenesis to replace the redox sensitive cysteine residues in the ZnS4 cluster with redox stable tetra-arginines.
They indicated that the extent to which the woodlands have changed in the densely populated and highly utilized site compared with the neighbouring two sites is a cause for concern.
Similar results were reported by Mapaure and Ndeinoma (2011) who demonstrated that utilization pressure has negatively impacted on the species composition and diversity of woodlands in heavily utilized site in Namibia compared with less utilized and protected Game Park.
To test this hypothesis, we utilized site directed mutagenesis to convert the consensus 'GATA' sequence to 'CAAA' in the context of a full length (3.0 Kb) gpdh-1 promoter for all three predicted GATA binding sites (Figure 4A).
Previous studies have utilized site directed mutagenesis to explore the amino acids involved in the active site and have demonstrated significant reductions in the enzyme activity associated with mutagenesis of these active site residues [14], [17].
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We utilized sites that were both significantly associated with Al tolerance differences in the association panel and sites not significantly associated, taking advantage of the difference in genetic resolution between association and linkage mapping.
All utilized sites were divided into 4 geographical regions: North (9 sites), East (12 sites), Central (14 sites), West (12 sites).
Three scheduling policies at grid level which utilize site load information are examined.
The too-robust hypothesis is often the implicit working hypothesis for those utilizing site directed mutagenesis to study protein mechanisms.
Some of these strategies utilize site specific recombination systems for the removal of superfluous DNA from the genome of genetically engineered plants [ 12, 22, 23].
The proposed model utilizes site-dependent failure probabilities and random link disruptions.
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