Sentence examples for utilized primer from inspiring English sources

Control experiments using Annexin utilized primer sequences F: 5'-TCTCGTTTTGCAATCTTGGCGTAT-3'; R: 5'-GGGTGCACGTGATGAGTCTCTT-3' and for Six1/2 F: 5'-GCTTCAAGGAGAAGTCGCGTGT-3'; R: 5'-TGCCTCCGATTTTTGAACCAGT-3'.

For the purposes of pyrosequencing, reverse primer 1100R was taken, following the literature that has utilized primer 530F for pyrosequencing analysis [ 21].

PCR amplification utilized primer pair Fovea2 and AdPr (TATG-ACA-CGC-GTC-GAC-TAGC) with DNA polymerase (Platinum® Taq DNA Polymerase; Life Technologies).

To test this possibility, we utilized primer pair F2/R1 to simultaneously amplify different-sized fragments from NANOG and NANOGP8 (Table 1) to screen for the possible presence/absence of NANOGP8 in 119 geographically diverse individuals from the entire Coriell SNP500Cancer and Africans South of the Sahara panels.

For the detection of ETEC, EPEC, EIEC, and EHEC initially three assays, n1, n2 and n3 were used as published earlier with some modifications [ 9]. Assay n1 utilized primer pairs for genes coding for heat stable toxin ST (stIa) and heat labile toxin LT (elt) of ETEC, and (uidA) for the E.coli β-glucuronidase.

This procedure utilized primers composed of 20 bases of known sequence with 8 random bases at the 3′-end that also served as an identifying barcode that allowed the differentiation each viral library following pooling and sequencing.

Nucleic acid extractions, PCR conditions, utilized primers, and sequence alignment procedures are as described in Betancur-R. et al. [23].

The first PCR reaction utilized primers 5'-CTAGTCCTAGGATCACCTCTCCGT-3' and 5'-GCTGACCACTGACCACAAGG-3' to amplify 6.9 kb of Cdc25B genomic DNA beginning within intron 1 and extending into intron 14.

The second PCR reaction utilized primers 5'-CCACCCTAGGCTATCTTTGC-3' and 5'-CCTAGGAAATGAGACTCATAC-3' to amplify 4.5 kb of Cdc25B genomic DNA beginning within intron 14 and extending to noncoding sequences downstream of exon 16.

25 µl RT-PCR and PCR reactions utilized primers listed in Table 1 with the predicted product sizes of 185 bp for vpu, 220 bp for vpr, 285 bp for vif, 320 bp for nef, and 380 bp for gag.

The lack of amplification bias was demonstrated for each utilized primer-pair by mixing different relative amounts of human placental DNA (Bioline, Taunton, MA) that had been methylated (with SssI-methyltransferase) and amplified DNA left unmethylated (HGHM5 and HGUM5, EpigenDx).