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All sequences utilized in this study, as well as the chopped boundaries utilized for PSSM generation, are available upon request.
A 1 30 ratio of defatted meal to solvent, and an extraction time of 120 min were utilized in this study, as recommended by Diosady et al. (2005).
The shape of crest and trough and therefore the shape of the annual cycle is the product of aquifer character, recharge and discharge quantum and its pattern and have been utilized in this study as a tool to arrive in for genetic classification of hard rock aquifer (see the supplementary sheets-1 & 2).
The Adaptive Grid Refinement in Fortran (AGRIF) deviation of ROMS (Penven et al., 2006; Debreu et al., 2011) was utilized in this study, as it allows the adaptive mesh refinement (AMR) nesting technique that is extendable to increase the resolution around domains of interest.
It is worth noting that, although the effort-moderated IRT model proposed in Wise and DeMars ([2006]) was based on the idea of the third approach, it was first utilized in this study as a validity check for the identified RT thresholds.
In major and minor salivary glands of the five adult subjects tested, utilized in this study as a control group, at variance with prenatal developmental stages, the pattern of reactivity for Tβ4 was characterized by the absence of granular deposits.
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The dataset utilized in this study is as compact as possible to minimize computer run times while still representing all of the known Hox-related gene families of Cnidaria, Deuterostomia, and Protostomia.
Cloning strategies to generate the various mutants utilized in this study were as follows: For pET15b/eIF4AIIG, two PCR products were produced using pET15b/eIF4AI as template and the primer pairs: (i) DraIII Forward and IG Reverse and (ii) IG Forward and BamHI Reverse (Table S1).
Two impeller rotation speeds were utilized in the study, as well as several angular and radial transducer positions at design, and at off-design flow rates.
No immunosuppressant such as cyclosporine A was utilized in the study as well as previous studies since cyclosporine A reduces glial response and exerts neuroprotection in experimental stroke.
The most important finding of the present study is that the low-field MRI method as utilized in this study is not yet as precise as the golden standard RSA.
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