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Using in silico primers and probes designed by a pattern identification method [14], our method utilized highly sensitive and specific tests for Bacteroidetes and Firmicutes.
Furthermore, most studies of marsupial imprinted gene expression have not utilized highly sensitive assays, such as pyrosequencing, to measure allele-specific expression of imprinted genes; so leaky expression of the repressed allele could be more prevalent than previously believed.
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We further utilized a highly sensitive and specific stem-loop RT-PCR for quantitative analysis of the expression patterns of these novel miRNAs across the life span of S. japonicum.
As microfluidic technologies utilize highly miniaturized and complex chip-based systems, there is a general demand for miniaturized detection methods that are sensitive and selective, in particular also for fluorescence detection [ 9].
Moreover, fish AChE activity has been utilized as a highly sensitive biomarker response of freshwater pollution (de la Torre et al. 2002).
The structure can be designed to have strong variation in transmittance with the refractive index of external medium and, thus, can be utilized as a highly sensitive refractive index sensor for biomedical applications.
The silica-based nanomaterials have been utilized to develop highly sensitive biosensors and bioassays.
For CENTRAL a strategy similar to that used for MEDLINE was utilized, without the highly sensitive term for clinical trials.
This technology platform has been utilized to discover highly sensitive and specific protein markers for oral cancer diagnosis and prognosis by comparing the protein profiles of cancer cells [ 4, 5], tissues [ 6], plasma [ 7], and saliva[ 8, 9] with appropriate controls.
We have utilized this mechanism to demonstrate highly sensitive ZnO thin film and LCM based UV sensor.
The activity of all PKN proteins was monitored through the use of a highly sensitive off-chip mobility shift assay utilizing a LabChip EZ Reader II instrument (Caliper Life Sciences/PerkinElmer); this assay format has been described elsewhere [ 31].
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