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Data analysis utilized gating on the forward/side scatter to evaluate CD69 expression on lymphocyte, monocyte/macrophage and PMN cell subsets.
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Beyond gating, there persists the question on how best to utilize gated data.
The system utilizes gated ring oscillators (GROs) and two counters to integrate photon time-of-arrival.
For lungs, lower grey histograms present the FMO signal gated on alveolar macrophages (AM) (pink gate).
Cells were gated on the lymphocyte fraction.
The plot was gated on the live CD4+CD3+CD8− population.
CTLA+ and CD25+ CD27− populations were gated on the CD4+ cells.
After 4 weeks of vector injection, erythroid subpopulations at progressive stages of differentiation were isolated from the marrow of four mice overexpressing PlGF and control mice, utilizing flow cytometry and gating on Ter-119-positive erythroid cells, and sorting on the basis of CD44 staining and forward scatter 34.
In this study we utilized EKG gating to correlate the image acquisition with electromechanical end diastole to obtain functional information on the diabetic LV.
The lymphocytic gate was established by gating on forward and side scatter characteristics.
By gating on DX5+ cells (gating shown in Fig. a), we analysed the expression of NKG2D on DX5+ cells.
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