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Trizol reagent (Invitrogen Life Technologies) was utilized for total RNA extraction from cells and placenta tissues.
Aim 2: Determination of mRNA and protein expression levels for genes encoding IRS-1, -2 and glucose transporter proteinsm Kidney, liver and brain tissues will be utilized for total RNA extraction using the Qiagen RNeasy Mini Kit [ 87- 89].
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A simple simulation showed that only the first part of the membrane was utilized for lower total feed flow rates and higher total absolute feed pressures.
Microwave digestion, wet digestion and dry ashing are commonly utilized for the total decomposition of organic matter in samples [11, 21, 22].
A classification approach for laboratory test outcome was utilized for a total of seven outcome variables shown in Table 1.
This new format is dissimilar from traditional PIHn in that the amount of polymer, the substrate on which the crystallizations occur, the volume of solvent utilized for crystallization, and the total amount of material used for the crystallization (∼1 mg) have been decreased dramatically.
Spectrophotometry and UPLC-DAD-ESI-MS/MS systems were utilized for quantitative analysis of total phenol, flavonoid and anthocyanin contents.
Histology and multi-photon microscopy were used for describing the media layer micro-architecture in the circumferential-radial plane, and Fourier Transform infrared imaging spectroscopy was utilized for determining structural protein, and total protein content.
When utilized for oil/water emulsion filtration, the total flux decline ratio of PVDF/PHFBM-PEGMA-PMTAC membrane was as low as 6.3% and the flux recovery ratio reached nearly 100%.
A single lane of an Illumina GAII run was utilized for each library and a total of more than 15 million 86-bp reads from each lane were produced.
One μg of total RNA was utilized for all hybridizations.
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