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The utilized primers are listed in Additional file 1. Primers utilized for sequence analysis of the MLH1 -93G>A polymorphism were designed to amplify the region spanning from -231 bp to -51 bp from the MLH1 transcription start site [ 39].
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The generated cDNA library was utilized for sequencing analysis using the Illumina Genome Analyzer (Illumina, San Diego, CA, USA), according to the manufacturer's instructions.
We thank Mark Dasenko for sequence analysis.
The rest sequences were then utilized for further analysis (assortment, alignment and tree-building) through the web servers of Influenza Virus Resource to elucidate their host and subtype distributions and identify the number of basic amino acid residues at the cleavage site in the HA sequences.
A custom oligonucleotide microarray (Affymetrix, Santa Clara, CA) based on the GBS COH1 genomic sequence [ 14] was utilized for microarray analysis.
The cDNA sequence alignment was further utilized for the analysis of positive selection using SELECTON (selecton.tau.ac.il) (Stern et al. 2007), as described (Gershoni et al. 2010).
High-quality sequence reads passing the Illumina purity filter (see footnote 8) were utilized for further analysis of variants after their adapter sequences were clipped using FastqMcf.
Among these technologies, bisulfite-modified whole-genome sequencing approaches have been utilized for methylome analysis at base pair resolution [ 35, 128].
Sequences with perfect match were utilized for further analysis.
Sequence Detector software (version 1.7) was utilized for data analysis.
This trend may be due to the models of sequence evolution utilized for estimating sequence divergence.
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