MS2 or product ion based quantitation strategies have been previously utilized for relative quantitation in isobaric tagging experiments and label-free techniques.
Soluble and alginate immobilized urease was utilized for detection and quantitation of mercury in aqueous samples.
Free as well as alginate immobilized urease was utilized for detection and quantitation of cadmium (Cd2+) in aqueous samples.
In addition, messenger RNA sequencing can be utilized for the identification of hybrid transcripts and quantitation of multiple isoforms resulting from alternative splicing.
This simple LC MS method achieves reliable quantitation and mass balance of 5-FdUrd, 5-FU, and their metabolites and can be effectively utilized for further kinetic studies.
Results were not utilized for therapeutic decisions.
COS7 cells were utilized for immunoprecipitation experiments.
For image quantitation, average R values were determined within multiple Regions of Interest (ROIs, single cells or small cell clusters) for each sample, and their Mean±SD (n = 7 to 9) determined and utilized for further statistical analysis (Student t-test).
Absolute signals of gene expression values generated on the different platforms could not be compared directly since the different platforms utilized different chemistry, quantitation, and normalization methods.
To confirm that the cell-death machinery was competent we utilized a cell-free system for quantitation of caspase activation.
