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The methodology was utilized for quantification of the GABA-A receptor α4-subunit, which is known to upregulate following withdrawal from chronic progesterone or neurosteroids.
The comparative 2−ΔCt method was utilized for quantification of each gene relative to GAPDH mRNA.
For instance, a chip-based, reversed-phase liquid chromatography-MALDI-MS analysis platform was utilized for quantification of glycomic-profile changes as cancer-biomarker discovery [ 32].
The principle of digital PCR was first introduced in the 1990s and is increasingly being utilized for quantification of DNA targets.
An Axioplan light microscope (Carl Zeiss, Oberkochen, Germany), equipped with an eyepiece graticule (grid), was utilized for quantification of MK numbers, per 20X magnification field, in 5 μm BM or spleen sections.
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Thus, we separately verified identities of peptides utilized for quantification by MS sequencing using LC-MS of endogenous neurexin proteins from mouse brain and immuno-precipitates obtained with a pan-NRX antibody.
An LC-MS/MS method for the separation of the two isomers was developed and utilized for quantification purpose.
Peak areas were integrated by MSD ChemStation (v. D.03.00.611) and utilized for quantification subsequent to their correction by both IS.
At the end of exposure period, cells were utilized for the quantification of cell-surface LH, prolactin, insulin receptors and their mRNA expression, the activity and mRNA expression of 11β-HSD and glucose oxidation.
Second, CAD may also be utilized for automated quantification of thrombus load [33], which is likely to lead to a significant reduction of the assessment time and therefore making evaluation of the thrombus load better applicable in the clinical setting.
A validated, liquid chromatography-tandem mass spectrometry method was utilized for the quantification of aprepitant plasma concentration in HIV-infected patients [ 45].
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