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The methodology was utilized for quantification of the GABA-A receptor α4-subunit, which is known to upregulate following withdrawal from chronic progesterone or neurosteroids.
The comparative 2−ΔCt method was utilized for quantification of each gene relative to GAPDH mRNA.
An LC-MS/MS method for the separation of the two isomers was developed and utilized for quantification purpose.
For instance, a chip-based, reversed-phase liquid chromatography-MALDI-MS analysis platform was utilized for quantification of glycomic-profile changes as cancer-biomarker discovery [ 32].
The principle of digital PCR was first introduced in the 1990s and is increasingly being utilized for quantification of DNA targets.
Peak areas were integrated by MSD ChemStation (v. D.03.00.611) and utilized for quantification subsequent to their correction by both IS.
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Here, we show experimentally that random-base molecular barcodes can be utilized for digital quantification of the absolute number of barcoded DNA molecules when using our specific barcode design and after computational analyses.
At the end of exposure period, cells were utilized for the quantification of cell-surface LH, prolactin, insulin receptors and their mRNA expression, the activity and mRNA expression of 11β-HSD and glucose oxidation.
As a routinely applied method for DNA analysis, UV was utilized for the quantification (OD260) and purity analysis for the plasmids.
Due to the near quantitative recovery of dissolved peptides with the FLU reagent and lack of analytical response to inorganic nitrogen, we proposed that FLU be utilized for the quantification of primary amine nitrogen.
Second, CAD may also be utilized for automated quantification of thrombus load [33], which is likely to lead to a significant reduction of the assessment time and therefore making evaluation of the thrombus load better applicable in the clinical setting.
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