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In targeted approaches, commonly employed to increase marker density in a specific chromosome region or to map genes of interest, only sequence data meeting specified parameters with respect to mapping or function are utilized for marker development.
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Barley chromosome 2H genome zipper (GZ) (Mayer et al. 2011) was utilized for initial marker development.
Upon mapping, the markers can be utilized for marker-assisted introgression of new germplasm into cultivated cotton and in subsequent breeding of agronomically adapted types, including cultivar development.
The polymorphisms were then utilized for marker-assisted backcross breeding programs (Bao et al. 2006).
Although clones for marker development were not chosen based on functional annotation, the sequence data utilized were known to be transcribed.
Sequence data for Miyazakimochi and Bikei 22 were used for marker development.
Except for high density, high polymorphism is the most important evaluation factor for marker development.
a The selected DNA polymorphisms in the GS5 promoter region for marker development.
Screen-captured images of IGV software showed nucleotide variations that were used for marker development.
a Identification of nucleotide polymorphisms in Gn1a gene for marker development.
Several techniques were used for marker development.
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