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Water-dipping objective lenses were utilized for live epifluorescent observation.
Our model is advantageous over commercially available 3D platforms as it can be utilized for live cell microscopy, and imaging live cell functional assays (such as proteolysis) [ 32].
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A two-stage cluster sampling process was utilized for those living alone (n = 110) and those living with family members (n = 102).
Cytotoxicity assay revealed that TPE NPs were biocompatible and thus can be utilized for long-term live cell imaging and biomedical applications.
Accordingly, the FbFP technology can be effectively utilized for long-term live cell bacterial imaging in the host cells including in animal models which are widely utilized by the researchers studying the local and systemic implications of chronic infections by opportunistic anaerobic pathogens.
A similar conclusion was reported by our group when a steroid-avoidance immunosuppression regimen was utilized for low-risk live-donor transplant recipients [ 3].
Importantly, the probe is successfully utilized for monitoring GSH in living HeLa cells.
What's more, through the fluorescence turn-on signal response to GSH, the probe was successfully utilized for monitoring GSH in living cells.
The "ON-OFF ON" fluON-OFF ONproperty ofluorescenton the addition of Al3+ and F− was utilized for the imaging of living cells.
What's more, cellular imaging experiment revealed that the sensor had excellent biocompatibility and low cytotoxicity that could be utilized for monitoring Hg2+ in living cells.
Optical and spectroscopic methods such as spectrophotometric methods [2], fluorescence microscopy [3] and confocal microscopy [4] can be utilized for the analysis of living cells, but have critical disadvantages due to the presence of exogenous fluorophores, which results in their providing limited information specific to only a small range of subcellular components.
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