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Sequences with perfect match were utilized for further analysis.
The rest sequences were then utilized for further analysis (assortment, alignment and tree-building) through the web servers of Influenza Virus Resource to elucidate their host and subtype distributions and identify the number of basic amino acid residues at the cleavage site in the HA sequences.
The uniquely mapped and PCR duplicates-removed reads, obtained using SAMTools [ 58], were utilized for further analysis.
High-quality sequence reads passing the Illumina purity filter (see footnote 8) were utilized for further analysis of variants after their adapter sequences were clipped using FastqMcf.
Since the ten bp observation window lacks sufficient read coverage (Additional file 1: Table S1), it was not utilized for further analysis due to lack of statistical significance.
Next, the ACGT101-miR program (version 4.2; LC Sciences, Hangzhou, China), a proprietary pipeline script, was utilized for further analysis [ 70].
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Also, it can be utilized for further binding analysis and screening of inhibitors of hPRR.
Gene Spring 7.3 (Agilent Technologies, USA), EDGE [ 26] and Microarray Software Suite-MeV4.0 http://www.tm4.org/ software were utilized for further data analysis and graphical display of results.
For image quantitation, average R values were determined within multiple Regions of Interest (ROIs, single cells or small cell clusters) for each sample, and their Mean±SD (n = 7 to 9) determined and utilized for further statistical analysis (Student t-test).
This comprehensive read depth could be utilized for further phylogenetic, strain and mutation analysis, relevant in microbial detection and research (Wang et al., 2007).
This method could thus be utilized for further osteoinductivity assessments.
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