Exact(6)
The primers utilized for expression analysis are shown in Table 1.
Furthermore, we strongly emphasize that reference genes utilized for expression profiling should be selected in a tissue specific manner.
Thus, for each of the constructs created in this study, 13 to 20 independent transformants were utilized for expression analysis.
Thus B-cells have been increasingly utilized for expression quantitative trait loci (eQTLs) studies [ 19, 24] and as a cell model to assess gene expression responses [ 25].
Global mRNA amplification has been employed in a range of applications, including more recent studies where microarrays have been utilized for expression profiling [ 6- 9].
We compared the expression profile of a specific gene by utilizing the TSS identified in this study with that of the same gene in which a previously identified TSS was utilized for expression.
Similar(54)
A TMV-based ICON expression vector was utilized for efficient expression of NaVCP.
E. coli strain BL21 (DE3) was transformed with expression plasmid pET28-σ and utilized for protein expression.
A previous report indicated that the osm-9 promoter we utilized for the expression in OLQ also drives the expression in AWA and ADL sensory neurons [ 35].
These cell lines have been utilized for PTTG1 expression control analysis since they exhibit different PTTG1 expression levels under normal culture conditions.
We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs.
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