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A Human Genome U133 Plus 2.0 array from Affymetrix, Inc. was utilized for each sample.
Only one sequencing lane of an eight lane flow cell was utilized for each sample, indicating that a cost-effective clinical test can be achieved.
The following PCR protocol was utilized for each sample and carried out in a Programmable Thermal Controller-100 (MJ Research, Inc., Waltham, MA): (1 94°CC × 12 minutes (polymerase activation), (2) thirty DNA polymerization cycles of 94°C × 45 seconds, 55°C × 45 seconds, 72°C × 45 seconds, followed by 72°C × 10 minutes and 4°C holding temperature.
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Here, the same berry samples from the same vineyard and climate, free of disease and insect pressures, were sampled and utilized for each Omic analysis.
For a nominal statistical power of 80%, the required sample size was utilized for each of these standardized effect sizes: 394, 64 and 26 per group, respectively.
Both the labeled and unlabeled samples are sufficiently utilized for each task, and the shared information between different tasks is simultaneously explored to facilitate decision making.
These data could also be utilized for determining sample size and estimating effect size in improving liver fat in weight loss studies in NASH/NAFLD.
To avoid the effect of inhomogeneities in the suspending medium, the background spectrum was taken using a sample from the same batch of phosphate buffered saline (PBS) utilized for the sample measurements.
In each window, 5 ns of MD simulation was performed so that a total simulation time of ∼200 ns was utilized for umbrella sampling in each system.
We consider a two-stage process, where the acquired information from Stage I (with a small sample size (m)) would be utilized for sample size (n) recommendation for Stage II study aiming for a more accurate estimation.
Three biological replicates were used for each analysis of adult cells, whereas five biological replicates were utilized for postnatal samples.
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