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Sweet potato with at least five fully expanded leaves per pot (n = 3) were utilized for each experiment, and each data set was from three biologically independent replicates.
PNST cells were cultured in 100mm tissue culture plates, and cells from 1 plate were utilized for each experiment when they reached 80-90% confluency.
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For each condition, 160 μg of protein sample was utilized for the experiment.
The frontal and neutral expression images from the ND Twins database were utilized for this experiment.
The concentrated methanol extract was partitioned, and the sequent partitioning, i.e., n-hexane (1.2 g), chloroform (800 mg), carbon tetrachloride (1.0 g), and aqueous soluble (1.6 g) fractions, was utilized for the experiment (Anosike et al. [2012]).
After 10 d the seedlings were utilized for the experiment.
The plants utilized for this experiment corresponded to BC5F4 whereas available IL seed stocks correspond to BC5F4 or BC5F5.
While photobleaching is not significant in G-CaMP over the period of time utilized for the experiment, it is significant for mDsRed.
The Affymetrix GeneChip ® Cotton Genome Array (Affymetrix Inc., Santa Clara, CA), containing 21,854 probe sets from four cotton species (G. arboreum, G. barbadense, G. hirsutum, and G. raimondii), was utilized for microarray experiment.
A poplar Nimblegen microarray http://www.nimblegen.com/ targeting 55,794 predicted genes from the Populus trichocarpa genome sequencing project, 126 mitochondrial and chloroplast gene models and 9995 unigenes derived from aspen EST sequences [ 25] were utilized for the experiment.
The remaining 110 specimens were utilized for the exposure experiment.
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