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The AUC studies utilized binding conditions identical to those used for the EMS experiments, except that the duplexes were labeled with 6-carboxyfluorescein (6-FAM).
The high resolution X-ray crystal structure of hCA II in complex with 5- 1-adamantylcarboxamido -1,3,4-thiadiazole-2-sulfonamide, showed the adamantyl moiety of the inhibitor residing in a less utilized binding pocket than that of most hydrophobic inhibitors, lined by the amino acid residues Ile91, Val121 and Phe131.
106 The most utilized binding agent is ethylene diamine tetra-acetic acid (ETDA), which can be included at a 15% concentration in a cream in association with topical steroids.
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In utilizing binding to a large receptor molecule, it may be important that the spike S1 domain also acts as a "spacer arm" holding the receptor far enough away from the three-fold axis of symmetry of the spike S2 domain to permit fusion core re-arrangement and subsequent membrane fusion.
By considering protein ligand interactions at the sub-cavity level, we can utilize binding information from structurally and functionally distinct proteins.
QacR is a member of the TetR family of repressors which share a helix−turn−helix DNA binding motif at their N-terminal regions but encode diverse C-terminal domains that are utilized in binding protein-specific inducing compounds (1– 3).
It is utilized in binding meat and fish and in gelled food products such as jelly, yogurt, and cheese.
As expected, all analyzed ligands utilized different binding modes with different amino acid residues involved due to structural and conformational mismatches.
We utilized ERα binding sites (ERαBS) located at the 20q13 region as the bait to interrogate the interactions between ERαBS and promoter regions of three loci.
Concordantly, the TCR residues utilized for binding were very similar in each case (Fig. 2E and F), but again more TCR residues were involved in binding to A2-EAA than A2-ELA.
To stably anchor gp140T to the surfaces of these particles under conditions preserving the antigenic integrity of the trimers, we utilized noncovalent binding of polyhistidine-tagged proteins to nitrilotri-acetic acid (NTA -headgroup lipids.
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