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To generate and apply pulsatile shear and circumferential stresses separately, a special custom-made bioreactor was utilized as previously described.
However, since the capsule utilizing the proposed protocol will be transmitting with lower transmission strength, the overall energy consumption will still be lower than when star topology TDMA is utilized as previously presented in Figure 11. Figure 15 Duty cycle vs. number of nodes.
Animal models were utilized as previously described [98].
Additional procedures to avoid DNA carry-over were utilized as previously described [7].
The CLP method was utilized as previously described [ 14].
Infective particles were produced and utilized as previously described.
Similar(46)
Human organ cultures of RPE/choroid were utilized as described previously Skeie et al., 2010.
To remove contaminating DNA, the Turbo DNA free kit (Ambion, Austin, USA) was utilized as described previously [ 45].
A Leica SP5 scanning confocal light microscope was utilized as described previously (Kelsey et al., 2012a; Kelsey et al., 2012b).
GAPDH was utilized as a reference gene as previously used in gene expression studies conducted in adipose tissues [10], [47], [61], [62], [63].
Alginate has been previously utilized as an adjuvant in nanoparticle-mediated gene delivery.
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